Different source atelocollagen thin films: Preparation, process optimisation and its influence on the interaction with eukaryotic cells

摘要

Collagen thin films were prepared via bovine atelocollagen matrices. The film casting was carried out by using different culture dishes, concentrations, equipment, drying processes and periods of time. In order to optimise the repeatability and reproducibility, microscopic analyses were utilised to explore the film quality and topographical patterning. In addition, the human immortalised non-tumorigenic keratinocyte cell line (HaCaT) was seeded onto the obtained specimens, and the cell proliferation was determined by using the MTT assay. These results indicated how the substrate, its concentration and processing conditions influence the cellular response. The attempted technique shows itself to be an excellent procedure for continuous collagen film preparation with optimal cell-proliferation rates, which may potentially be used in tissue engineering or wound-healing applications.