首页> 外文OA文献 >Acidiplasma aeolicum gen. nov., sp. nov., a euryarchaeon of the family Ferroplasmaceae isolated from a hydrothermal pool, and transfer of Ferroplasma cupricumulans to Acidiplasma cupricumulans comb. nov.
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Acidiplasma aeolicum gen. nov., sp. nov., a euryarchaeon of the family Ferroplasmaceae isolated from a hydrothermal pool, and transfer of Ferroplasma cupricumulans to Acidiplasma cupricumulans comb. nov.

机译:嗜酸菌十一月,sp。 11月,从热液池中分离出铁杆亚科的一个euryarchaeon,并将铁杆亚铁转移到铜亚铁梳中。十一月

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摘要

A novel acidophilic, cell-wall-less archaeon, strain V(T), was isolated from a hydrothermal pool on Vulcano Island, Italy. The morphology of cells was observed to vary from pleomorphic to coccoid. The temperature range for growth of strain V(T) was 15-65 degrees C with an optimum at 45 degrees C. The pH for growth ranged from pH 0 to 4 with an optimal at pH 1.4-1.6. Strain V(T) was able to grow aerobically and anaerobically, oxidizing ferrous iron and reducing ferric iron, respectively. The isolate grew chemo-organotrophically with yeast extract and yeast extract with glucose as the sources of energy and carbon. The molar G+C content in the DNA was 36 mol%. 16S rRNA gene sequence analysis demonstrated that strain V(T) was a member of the family Ferroplasmaceae, order Thermoplasmatales, phylum Euryarchaeota, showing sequence identities of 100 % with Ferroplasma cupricumulans BH2(T), 95.4 % with Ferroplasma acidiphilum Y(T), 94 % with Picrophilus torridus DSM 9790(T) and 92 % with Picrophilus oshimae DSM 9789(T). 16S rRNA gene sequence-based phylogenetic analysis showed that strain V(T) formed a monophyletic cluster together with F. cupricumulans BH2(T) and all other thermophilic isolates with available 16S rRNA gene sequences, whereas F. acidiphilum Y(T) formed another cluster with mesophilic isolates within the family Ferroplasmaceae. DNA-DNA hybridization values between strain V(T) and F. cupricumulans BH2(T) were well below 70 %, indicating that the two strains belong to separate species. Principal membrane lipids of strain V(T) were dibiphytanyl-based tetraether lipids containing pentacyclic rings. The polar lipids were dominated by a single phosphoglycolipid derivative based on a galactosyl dibiphytanyl phosphoglycerol tetraether, together with smaller amounts of monoglycosyl and diglycosyl dibiphytanyl ether lipids and the corresponding phosphoglycerol derivatives. The major respiratory quinones present were naphthoquinone derivatives. Given the notable physiological and chemical differences as well as the distinct phylogenetic placement of the new isolate relative to the type species of the genus Ferroplasma, we propose strain V(T) as a member of a new genus and species, Acidiplasma aeolicum gen. nov., sp. nov. The type strain of Acidiplasma aeolicum is strain V(T) (=DSM 18409(T) =JCM 14615(T)). In addition, we propose to transfer Ferroplasma cupricumulans Hawkes et al. 2008 to the genus Acidiplasma as Acidiplasma cupricumulans comb. nov. (type strain BH2(T) =DSM 16551(T) =JCM 13668(T)).
机译:从意大利Vulcano岛的热液池中分离出了一种新型的嗜酸,无细胞壁的古细菌V(T)。观察到细胞的形态从多形到类球体变化。菌株V(T)的生长温度范围为15-65℃,最适为45℃。生长的pH范围为pH 0至4,最适pH为1.4-1.6。菌株V(T)能够有氧和厌氧地生长,分别氧化亚铁和还原三价铁。该分离物以酵母提取物和葡萄糖为能源和碳源的酵母提取物在化学-营养上生长。 DNA中的G + C摩尔含量为36mol%。 16S rRNA基因序列分析表明,菌株V(T)是铁杆亚种,热丝亚目,Euryarchaeota门,显示与铜铁亚铁BH2(T)的序列同一性为100%,与嗜酸铁亚铁的Y(T)为95.4%, 94%的人用Picrophilus torridus DSM 9790(T),92%的人使用Picrophilus oshimae DSM 9789(T)。基于16S rRNA基因序列的系统发育分析表明,菌株V(T)与F. cupricumulans BH2(T)以及所有其他具有可用16S rRNA基因序列的嗜热菌株一起形成了单系簇,而F. acidiphilum Y(T)形成了另一个与铁杆科的嗜温菌分离。菌株V(T)和铜霉BH2(T)之间的DNA-DNA杂交值远低于70%,表明这两个菌株属于不同的物种。菌株V(T)的主要膜脂是含有五环的基于二联二氧萘基的四醚脂。极性脂质主要由基于半乳糖基二双植烷酰基磷酸甘油四醚的单一磷酸糖脂衍生物,少量的单糖基和二糖基二双植物丹脂醚脂质以及相应的磷酸甘油衍生物所主导。存在的主要呼吸醌是萘醌衍生物。鉴于显着的生理和化学差异,以及新分离株相对于铁质属类型物种的明显系统发生位置,我们建议菌株V(T)作为新属和物种Aeolicum aeolicum gen的成员。十一月,sp。十一月酸性嗜酸菌的类型菌株是菌株V(T)(= DSM 18409(T)= JCM 14615(T))。此外,我们建议转让Ferroplasma cupricumulans Hawkes等人。 2008年以酸枝杆菌为梳状酸枝杆菌。十一月(类型BH2(T)= DSM 16551(T)= JCM 13668(T)))。

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