Detección de circovirus porcino tipo 2 en cerdos del Ecuador mediante PCR.

摘要

This report describes the first detection of Porcine Circovirus Type 2 (PCV2) from pigs affected with and without Porcine Circovirus Associated Diseases (PCVA) in Ecuador. A total of 162 samples were collected during November 2010 and March 2011: 25 from commercial swine herds with a range of clinical signs and the rest randomly at slaughter houses. Using two sets of polymerase chain reaction (PCR) primers, it was possible to detect PCV2 DNA in 62.35% (101/162) of pigs. Positive PCR reactions (24/25) from samples of pigs with clinical signs suggest that PCV2 is associated with the symptoms of PCVA. Additionally, the nucleotide sequence analysis confirms the PCV2 detection with at least a 95% homology compared with other PCV2 found in the GenBank database. Previous to this study vaccines were not commercially available in Ecuador. With these results, the Sanitary Institution in Ecuador has extended the importation permission.