Avaliação da citotoxicidade, liberação de monômero residual, sorção e solubilidade em água de resinas compostas

摘要

This study aimed to evaluate the toxicity of composite resins through an ―in vitro‖ cytotoxicity test, as well as, to determine the water sorption, solubility and released monomers. The samples were divided into 3 groups: nanofiller composite resin (Supreme), nanohybrid composite resin (Esthet-X) e microhybrid composite resin (4seasons). Only one resin composite increment was placed into teflon molds (3mm diameter and 2mm high) and was photopolymerized. Each material was divided into 2 subgroups according curing light unit used to photopolymerized composites. Water sorption and solubility measurements were obtained by means of weighting the samples before and after water immersion and desiccation. To quantify the residual monomers released from composites, using ultraviolet spectrophotometry (UV). The cytotoxicity assay was performed by fibroblast culture (NIH/3T3 line) in complete D-MEM. With a confluence of 80% the suspension was added on the plaques of 24 wells with the samples and incubated at 37°C for 24, 48, 72 and 168 hours. The cell viability was quantified by MTT assay. The values were statistically analyzed and the results revealed that light curing unit did not influence water sorption and solubility. On the other hand, monomers release and cytotoxicity were influenced by photopolymerization. The different periods evaluated interfered only for leaching monomers, with maximal concentration at the 3-day period. We concluded that all composites demonstrated modification after water immersion, different ranges of monomers releasing and cytotoxicity. Thus, the monomers release and cytotoxicity decreased with composite resin were photopolymerized by LED.