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Fatty acid and conjugated linoleic acid isomer profiles in human milk fat

机译:人乳脂肪中的脂肪酸和共轭亚油酸异构体谱

摘要

The fatty acid composition of 39 mature human milk samples from four Spanish women collected between 2 and 18 weeks during lactation was studied by gas chromatography. The conjugated linoleic acid (CLA) isomer profile was also determined by silver-ion HPLC (Ag+-HPLC) with three columns in series. The major fatty acid fraction in milk lipids throughout lactation was represented by the monounsaturated fatty acids, with oleic acid being the predominant compound (36-49% of total fatty acids). The saturated fatty acid fraction represented more than 35% of the total fatty acids, and polyunsaturated fatty acids ranged on average between 10 and 13%. Mean values of total CLA varied from 0.12 to 0.15% of total fatty acids. The complex mixture of CLA isomers was separated by Ag+-HPLC. Rumenic acid (RA, cis-9 trans-11 C18:2) was the major isomer, representing more than 60% of total CLA. Trans-9 trans-11 and 7-9 (cis-trans + trans-cis) C18:2 were the main CLA isomers after RA. Very small amounts of 8-10 and 10-12 C18:2 (cis-trans + trans-cis) isomers were detected, as were different proportions of cis-11 trans-13 and trans-11 cis-13 C18:2. Although most of the isomers were present in all samples, their concentrations varied considerably. © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
机译:通过气相色谱法研究了在哺乳期2至18周之间收集的来自四名西班牙妇女的39份成熟人乳样品的脂肪酸组成。共轭亚油酸(CLA)异构体谱图也通过三列串联的银离子HPLC(Ag + -HPLC)测定。整个泌乳期乳脂中的主要脂肪酸部分以单不饱和脂肪酸为代表,其中油酸为主要化合物(占总脂肪酸的36-49%)。饱和脂肪酸部分占总脂肪酸的35%以上,多不饱和脂肪酸的平均范围为10%至13%。总CLA的平均值为总脂肪酸的0.12%至0.15%。通过Ag + -HPLC分离CLA异构体的复杂混合物。瘤胃酸(RA,cis-9 trans-11 C18:2)是主要的异构体,占总CLA的60%以上。反式9反式11和7-9(顺式-反式+反式-顺式)C18:2是RA后的主要CLA异构体。检测到非常少量的8-10和10-12 C18:2(顺式-反式+反式-顺式)异构体,以及不同比例的顺式11反式13和反式11顺式13 C18:2。尽管大多数样品中都存在大多数异构体,但它们的浓度差异很大。 ©2007 WILEY-VCH Verlag GmbH&Co. KGaA,Weinheim。

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