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Regulation of peripheral classical and non-classical monocytes on infliximab treatment in patients with rheumatoid arthritis and ankylosing spondylitis.

机译:类风湿关节炎和强直性脊柱炎患者对英夫利昔单抗治疗的外周经典和非经典单核细胞的调节。

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摘要

OBJECTIVEududTo investigate the regulatory effect of tumour necrosis factor (TNF) blockade with infliximab on the distribution of peripheral blood monocyte subpopulations in patients with rheumatoid arthritis (RA) and ankylosing spondylitis (AS).ududMETHODSududPurified CD11b+CD14+ monocytes from 5 patients with RA and 5 AS were analysed ex vivo before and after infliximab treatment by flow cytometry for CD16, CD163, CD11b, C-C chemokine receptor type 2 (CCR2) and CXC chemokine receptor 4 (CXCR4) at baseline and at days 2, 14, 84 and 168 after the first infliximab administration. Serum levels of the stromal cell-derived factor (SDF)-1 and monocyte chemotactic peptide (MCP)-1 at different time points were measured in either patient group before and on infliximab treatment.ududRESULTSududAnti-TNF treatment with infliximab led to a significant increase of circulating CD11b+ non-classical and a concomitantly decrease of CD11b+ classical monocytes, to a decline in SDF-1 levels and reduced expression of CCR2 and CXCR4 on non-classical monocyte subpopulation.ududCONCLUSIONSududOur study shows, that TNFα blockade by infliximab resulted in a dichotomy of the regulation of classical and non-classical monocytes that might have substantial impact on inhibition of osteoclastogenesis and of subsequent juxta-articular bone destruction and systemic bone loss in RA and AS.
机译:目的 ud ud研究用英夫利昔单抗阻断肿瘤坏死因子(TNF)对类风湿关节炎(RA)和强直性脊柱炎(AS)患者外周血单核细胞亚群分布的调节作用。 ud udMETHODS ud udPurified在英夫利昔单抗治疗之前和之后,通过流式细胞术对来自5名RA和5名AS患者的CD11b + CD14 +单核细胞进行了离体分析,分别在基线和在首次英夫利昔单抗给药后第2、14、84和168天。在英夫利昔单抗治疗前后,在任一患者组中测量了不同时间点的基质细胞衍生因子(SDF)-1和单核细胞趋化肽(MCP)-1的血清水平。 ud udRESULTS ud ud抗TNF治疗英夫利昔单抗联合使用导致非经典单核细胞亚群中循环CD11b +的显着增加以及CD11b +经典单核细胞的减少,导致SDF-1水平下降,CCR2和CXCR4的表达降低。 ud我们的研究表明,英夫利昔单抗对TNFα的阻断导致对经典和非经典单核细胞调节的二分法,这可能对抑制破骨细胞生成以及随后的RA和AS的近关节骨破坏和全身性骨丢失产生重大影响。

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