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Microbiological study of showerhead waters from 10 beaches of great Lisboa and West Regions of Portugal

机译:葡萄牙新葡京及西部地区10个海滩的莲蓬头水的微生物学研究

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摘要

Many bacteria pathogenic to man can interfere in water distribution systems, forming aggregates that give rise to a complex structure called biofilm. In order to monitor the microbiological content of water from beach showers from ten sites along the coast from Lisboa-Carcavelos to Foz do Arelho beaches, 24 water samples were analyzed in 2013, 12 during January and February, and other 12 during June. The samples were collected in 500 mL sterile bottles with sodium thiosulfate. Biofilms were collected on swabs (at the same water sampling points) with transport medium Tween 20, and maintained between 2 ºC and 4 ° C, such as water samples. The following microbiological parameters were investigated for water samples: total microbial count at 22 ºC and 37 ºC, total coliforms and Escherichia coli, Pseudomonas aeruginosa, fecal streptococci, total Staphylococcus and coagulase-positive Staphylococcus. The swabs lead to the isolation of the microorganisms grown on the nutrient agar medium, in order to determine the the same microbiological parameters investigated in water showers. Globally, the water quality was found to be suitable, but that does not rule out the need for constant monitoring of the beach showers. Taking into account that there have been reports of plankton invasions and allergic eruptions after bathing in some seaside spots near Lisboa, these study results prompt us to recommend that swimmers should always shower after bathing or diving since showerheads were found safe all over the coast.
机译:许多对人类有致病性的细菌会干扰水分配系统,形成聚集体,从而形成称为生物膜的复杂结构。为了监测从里斯本-卡尔卡维卢斯到福斯-多阿雷霍海滩沿岸十个地点的海滩淋浴水的微生物含量,2013年分析了24个水样,一月和二月分析了12个水样,6月分析了另外12个水样。将样品收集在装有硫代硫酸钠的500 mL无菌瓶中。生物膜被收集在棉签(在相同的水采样点)上,并带有Tween 20转运介质,并保持在2ºC至4ºC之间,例如水样。对水样品进行了以下微生物学参数研究:22℃和37℃下的总微生物数,大肠菌群和大肠杆菌,铜绿假单胞菌,粪便链球菌,总葡萄球菌和凝固酶阳性葡萄球菌。拭子导致了在营养琼脂培养基上生长的微生物的分离,以便确定在水淋中研究的相同微生物参数。在全球范围内,发现水质是合适的,但这并不排除需要对海滩淋浴进行持续监测的情况。考虑到有报道称在里斯本附近的一些海滨地区沐浴后浮游生物入侵和过敏爆发,这些研究结果促使我们建议游泳者在沐浴或潜水后应始终淋浴,因为在整个沿海地区都发现了莲蓬头是安全的。

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