Metabolism of androstenone, 17β-estradiol and dihydrotestosterone in primary cultured pig hepatocytes and the role of 3β-hydroxysteroid dehydrogenase in this process

摘要

Steroids metabolism plays an important role in mammals and contributes to quality of pig meat. The main objective of this study was to identify metabolites of androstenone, 17b-estradiol and dihydrotestosterone using primary cultured pig hepatocytes as a model system. The role of 3b-hydroxysteroid dehydrogenase (3bHSD) in regulation of steroid metabolism was also validated using trilostane, a specific 3bHSD inhibitor. Steroid glucuronide conjugated metabolites were detected by liquid chromatography time of flight mass spectrometry (LC-TOF-MS). 3bHSD enzyme was essential for metabolism of androstenone to 5a-androst-16-en-3b-ol, which then formed androstenone glucuronide conjugate. Metabolism of 17bestradiol was accompanied by formation of glucuronide conjugated estrone and glucuronide-conjugated estradiol. The ratio of the two metabolites was around 5:1. 3bHSD enzyme was not involved in 17b-estradiol metabolism. 5a-Dihydrotestosterone-17b-glucuronide was identified as a dihydrotestosterone metabolite, and this metabolism was related to 3bHSD enzyme activity as demonstrated by inhibition study.