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>Response Surface Methodology for Optimization of the Erythromycin Production by Fed-Batch Fermentation Using an Inexpensive Biological Nitrogen Source
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Response Surface Methodology for Optimization of the Erythromycin Production by Fed-Batch Fermentation Using an Inexpensive Biological Nitrogen Source
A novel source of biological nitrogen produced by multi-strain fermentation was developed for erythromycin production.The nitrogen composition of corn steep liquor, biological nitrogen and soybean flour was optimized by using Response surface methodology (RSM) firstly. A Box-Behnken design was used to estimate the optimum nitrogen composition for the production of erythromycin as follows: corn steep liquor: γ =5.1 g L-1, biological nitrogen γ = 5.96 g L-1 and soybean flour γ = 24.17 g L-1.Based on the optimum nitrogen source, the constant glucose fed-batch and pH control fed-batch strategies were used to further optimize the erythromycin production in 50 L stirred bioreactor, respectively.It was found that pH control could serve as an effectivecontrol strategy for erythromycin production, and the maximum erythromycin production was 8528 U mL-1 at 190 h.This work demonstrates that the new medium formulation based on a cheap nitrogen source, biological nitrogen, is a potential alternative foreconomic erythromycin production on a large scale.
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机译:为红霉素的生产开发了一种新的多菌种发酵产生的生物氮源。首先采用响应面法(RSM)优化了玉米浆,生物氮和大豆粉的氮组成。使用Box-Behnken设计来估算生产红霉素的最佳氮组成,如下所示:玉米浆:γ= 5.1 g L-1,生物氮γ= 5.96 g L-1,大豆粉γ= 24.17 g L -1。基于最佳氮源,分别采用恒定葡萄糖补料和pH控制补料策略分别优化50 L搅拌生物反应器中红霉素的产量,发现pH控制可作为有效的控制方法。 190 h时,最大红霉素产量为8528 U mL-1。这项工作表明,基于廉价氮源(生物氮)的新培养基配方是大规模生产红霉素的潜在替代品。
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