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A comparison of indirect methods for diagnosis of subclinical mastitis in lactating dairy cows

机译:间接诊断泌乳奶牛亚临床乳腺炎的方法比较

摘要

A total of 1014 samples taken from Lithuanian cows at the end of milking were tested on several parameters: milk reaction with Mastest (developed, tested, and produced at the Lithuanian Veterinary Institute), California Mastitis Test (CMT), Bernburg, Profilac reagent, Eimü-milchtest. Somatic cell count (SCC) was established using the flow cytometry method and Anadis F device; electrical conductivity (EC) was measured using a Mastiidiindikaator device made in Estonia. Electrical conductivity of the samples taken from healthy cows was 4.5-6.0 mS/cm, while EC in the samples taken from cows with subclinical mastitis was 6.1-8.5 mS/cm. Even though electrical conductivity of milk produced by the cows with mastitis tends to increase, it does not always correlate with SCC. Mastest results correlate best (95.5-100%) somatic cell count in milk when reaction of milk with milk is rated to be 1, 2, and 5 points, while the poorest correlation (87.62%) is at a rating of 3 points. Mast test results correlate well with those of other tests. Total coefficient of correlation between Mastest and Bernburg reagent r = +0.985, between Mastest and Profilac r = +0.980, between Mastest and Eimü-milchtest r = +0.966. The best correlation (99.39-100%) between Mastest and CMT is at a rating of 1 and 5 points. At the intermediary ratings (2-4 points) test results correlate 95.96-99.05%. Overall correlation coefficient r = +0.997.
机译:在挤奶结束后从立陶宛奶牛中采集的总共1014个样品在以下几个参数上进行了测试:与Mastest的牛奶反应(立陶宛兽医研究所开发,测试和生产),加利福尼亚乳腺炎测试(CMT),伯恩堡,Profilac试剂, Eimü-milchtest。使用流式细胞仪和Anadis F装置建立体细胞计数(SCC)。使用爱沙尼亚制造的Mastiidiindikaator装置测量电导率(EC)。取自健康母牛的样品的电导率为4.5-6.0 mS / cm,而取自亚临床乳腺炎的母牛的样品的EC为6.1-8.5 mS / cm。即使患有乳腺炎的奶牛生产的牛奶的电导率趋于增加,但并不总是与SCC相关。当将牛奶与牛奶的反应定为1、2和5分时,Mastest结果与牛奶中的体细胞计数最佳相关(95.5-100%),而最差的相关性(87.62%)与3分相关。桅杆测试结果与其他测试的相关性很好。 Mastest与Bernburg试剂之间的总相关系数r = + 0.985,Mastest与Profilac之间的r = +0.980,Mastest与Eimü-milchtest之间的r = +0.966。 Mastest与CMT之间的最佳相关性(99.39-100%)为1分和5分。在中间等级(2-4分)下,测试结果与95.96-99.05%相关。总体相关系数r = +0.997。

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