Cultivable microorganisms associated with honeys of different geographical and botanical origin

摘要

In this study, the composition of the cultivable microbial populations of 38 nectar honey and honeydewudhoney samples of different botanical and geographical origin were assessed. After growth in specificudmedia, various colonies with different appearance were isolated and purified before phenotypicud(morphological, physiological and biochemical traits) and genotypic [randomly amplified polymorphicudDNA (RAPD), repetitive DNA elements-PCR (rep-PCR) and restriction fragment length polymorphismud(RFLP)] differentiation. The identification was carried out by 16S rRNA gene sequencing for bacteria and,udin addition to RFLP, by sequencing the D1/D2 region of the 26S rRNA gene for yeasts and the 5.8S-ITSudrRNA region for filamentous fungi. The results showed the presence of 13 species of bacteria, 5 of yeastsudand 17 of filamentous fungi; the species most frequently isolated were Bacillus amyloliquefaciens,udZygosaccharomyces mellis and Aspergillus niger for the three microbial groups, respectively. The highestudmicrobial diversity was found in multifloral honeys. No correlation among the microbial species and theudbotanical/geographical origin was found, but some strains were highly adapted to these matrices sinceudthey were found in several samples of different origin.