Capillary electrophoretic and extraction conditions for the analysis of Catha edulisudFORKS active principles

摘要

A capillary electrophoretic method, which allowed the detection and separation of the active principlesudof Catha edulis, i.e. cathinone, cathine and phenylpropanolamine, was developed. A suitable internaludstandard (nicotinamide), which permitted the quantification of the analytes reducing the variability ofudthe migration times due to EOF changes, was identified. The analytical method was validated, assessingudlinearity, sensitivity and repeatability, showing optimal features for the analysis of the vegetableudmaterial. Moreover extraction conditions were investigated to achieve the exhaustion of the plantudmaterial in the fastest and most efficient way to meet the requirements of the Court.