From targeted analysis to untargeted metabolomics and its application in food and nutritional studies

摘要

Metabolomics is applied to a variety of biological fields from medical science to agriculture. Most of human beneficial properties of plants, be they foods, medical resources, or industrial raw materials, are ascribed to plant metabolites. One of the most important class of compounds are the polyphenols, due to compelling evidences of their beneficial health properties and their impact on food quality. The complexity and remarkable diversity of polyphenols has challenged the analytical performances of the separation and detection methods in terms of resolving power, selectivity and sensitivity required for the identification and quantification of these compounds in different matrices.Targeted metabolomics is a strategy based on the use of predefined metabolite-specific signals, such as MRM transitions in the case of tandem mass spectrometry, that can be used to accurately and selectively determine the concentrations of a wide range of known metabolites, over a broad dynamic range. A targeted metabolomics method using UPLC/MS/MS system has been developed for the quantification of >150 polyphenols and the catabolites associated with the consumption of polyphenols-rich fruits. The validated method was found to be particularly flexible, since it can be easily expanded in different experiments. It was successfully applied to the analysis of various fruits, as well as in nutritional studies, providing a valuable tool for the metabolite profiling of both the native compounds present in food and some nutritionally important bioactive catabolites in biofluids and organs.For a mechanistic understanding of the action of polyphenols in living organisms it is fundamental in nutritional studies to include also untargeted metabolomics, a powerful tool to study processes in organisms and to detect new biologically important biomarkers. In our study untargeted high resolution mass spectrometry-based investigation was chosen to monitor the metabolic effects induced by administration of different dietary polyphenols, at a physiologically relevant dose, in rats