The Latest Information and Perspective of Human Gene and Protein Database (HGPD) as Human Proteome Study

摘要

HGPD ("http://www.HGPD.jp/":http://www.HGPD.jp/) is a unique database that stores information on a set of human Gateway entry clones in addition to protein expression data. HGPD was launched in November 2008 and, since then, 33,275 human Gateway entry clones have been constructed from ORFs of full-length cDNAs, thus representing the largest collection in the world. Sequence information and protein expression of Gateway entry clones can also be retrieved from HGPD. The majority of analysis data for cDNA sequences in HGPD are shared with the FLJ Human cDNA Database ("http://flj.hinv.jp/v01/cgi/index.cgi":http://flj.hinv.jp/v01/cgi/index.cgi), which was constructed as human cDNA sequence analysis database focusing on mRNA varieties resulting from variations in transcription start site (TSS) and splicing. In this update, we constructed an additional 9,974 Gateway entry clones, giving a total of 43,249, corresponding to approximately 2,000 loci. Sequence summary, nucleotide and amino acid sequence data for these clones (v 2.0) can be obtained from download site. We named this human Gateway entry clone resource “HUPEX” (Human Proteome Expression-resource). And, we expressed 4,457 human proteins with a C-terminal V5 and His tag and analyzed them using SDS-PAGE, giving a total of 17,821. In the near future, we will construct 11,000 image data of subcellular localization of the proteins with fluorescent proteins at the N- or C-terminal which are expressed in HeLa cells by using Gateway entry clones. And, we will link ESPRESSO ("http://mbs.cbrc.jp/ESPRESSO/":http://mbs.cbrc.jp/ESPRESSO/) using ORF sequences of the entry clones, allowing us to acquire predictional protein expression and solubility results. Moreover, we will redesign the web interface.