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Human heart failure biomarker immunosensor based on excessively tilted fiber gratings

机译:基于过度倾斜的光纤光栅的人心衰竭生物标志物免疫传感器

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摘要

A label-free immunosensor platform based on excessively tilted fiber gratings (Ex- TFGs) was developed for highly specific and fast detection of human N-terminal pro-B-type natriuretic peptide (NT-proBNP), which is considered a powerful biomarker for prognosis and risk stratification of heart failure (HF). High-purity anti-NT-proBNP monoclonal antibodies (MAbs) prepared in our laboratory were immobilized on fiber surface through the staphylococcal protein A (SPA) method for subsequent specific binding of the targeted NTproBNP. Utilizing fiber optic grating demodulation system (FOGDS), immunoassays were carried out in vitro by monitoring the resonance wavelength shift of Ex-TFG biosensor with immobilized anti-NT-proBNP MAbs. Lowest detectable concentration of ~0.5ng/mL for NTproBNP was obtained, and average sensitivity for NT-proBNP at a concentration range of 0~1.0 ng/mL was approximately 45.967 pm/(ng/mL). Several human serum samples were assessed by the proposed Ex-TFG biomarker sensor, with high specificity for NT-proBNP, indicating potential application in early diagnosing patients with acute HF symptoms.
机译:开发了基于过度倾斜的光纤光栅(Ex-TFGs)的无标记免疫传感器平台,用于高特异性和快速检测人N末端前B型利尿钠肽(NT-proBNP),后者被认为是一种强大的生物标记心力衰竭(HF)的预后和风险分层。通过金黄色葡萄球菌蛋白A(SPA)方法将在我们实验室中制备的高纯度抗NT-proBNP单克隆抗体(MAbs)固定在纤维表面,以用于随后与目标NTproBNP的特异性结合。利用光纤光栅解调系统(FOGDS),通过用固定的抗NT-proBNP MAb监测Ex-TFG生物传感器的共振波长偏移,在体外进行免疫测定。 NTproBNP的最低可检测浓度为〜0.5ng / mL,在0〜1.0 ng / mL的浓度范围内,NT-proBNP的平均灵敏度约为45.967 pm /(ng / mL)。通过拟议的Ex-TFG生物标志物传感器评估了几种人血清样品,这些样品对NT-proBNP具有高度特异性,表明其在早期诊断急性HF症状的患者中具有潜在的应用价值。

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