Crystallization and preliminary diffraction studies of firefly luciferase from $Photinus$ $pyralis$

摘要

Firefly luciferase is a 62 kDa molecular weight enzyme which catalyzes a light-emitting reaction. Crystals of Photinus pyralis luciferase have been obtained by the microbatch technique, using polyethylene glycol as a precipitating agent. Firefly luciferase crystallizes as long needles which belong to the tetragonal space group P41212, with cell dimensions a = 119.5, b = 119.5, c = 95.4 Å. One molecule is present in the asymmetric unit. Diffracted intensities beyond 2.0 Å resolution have been measured from frozen crystals using synchrotron radiation.