High frequency plant regeneration from mature seed of elite, recalcitrant Malaysian indica rice (Oryza sativaL.) CV. MR 219

摘要

An efficient in vitro plant regeneration system was established for elite, recalcitrant Malaysian indica rice,udOryza sativa L. CV. MR 219 using mature seeds as explant on Murashige and Skoog and Chu N6 mediaudcontaining 2,4-dichlorophenoxy acetic acid and kinetin either alone or in different combinations.udL-proline, casein hydrolysate and L-glutamine were added to callus induction media for enhancement ofudembryogenic callus induction. The highest frequency of friable callus induction (84%) was observedudin N6 medium containing 2.5 mg lud–1 2,4-dichlorophenoxy acetic acid, 0.2 mg lud–1 kinetin, 2.5 mg lud–1udL-proline, 300 mg l-1 casein hydrolysate, 20 mg lud–1 L-glutamine and 30 g lud–1 sucrose under culture inudcontinuous lighting conditions. The maximum regeneration frequency (71%) was observed, whenud30-day-old N6 friable calli were cultured on MS medium supplemented with 3 mg lud–1 6-benzyl aminopurine,ud1 mg lud–1 naphthalene acetic acid, 2.5 mg lud–1 L-proline, 300 mg lud–1 casein hydrolysate and 3% maltose.udDeveloped shoots were rooted in half strength MS medium supplemented with 2% sucrose and wereudsuccessfully transplanted to soil with 95% survival. This protocol may be used for other recalcitrantudindica rice genotypes and to transfer desirable genes in to Malaysian indica rice cultivar MR219 for cropudimprovement.