Role of anti‐transglutaminase (anti‐tTG), anti‐gliadin, and anti‐endomysium serum antibodies in diagnosing celiac disease: A comparison of four different commercial kits for anti‐tTG determination

摘要

The aims of this study were: (1) to compare the diagnostic efficacy for celiacuddisease (CD) diagnosis of serum determination of anti-gliadin (AG) (IgA and IgG) udand anti-endomysium (AE) with that of anti-transglutaminase (AtTG); and (2) toudcompare the accuracy of four different assays to measure AtTG. We studied 72udchildren: the histological diagnosis of CD was made in 38 cases and excluded inudthe remaining 34 children. In fasting sera we measured AE, AG-IgA and IgG, andudAtTG, the latter with four different commercial kits (Eurospital, Medipan, Inova,udArnika). Moreover AtTG was measured in a group of 58 CD children after audgluten-free diet. AE was positive in all but 1 case of CD patients (sensitivity =ud97%); false positive results were found in 1/34 controls (specificity = 97%).udWhen a specificity of 95% was fixed, the sensitivities were 97% for AE, 83% forudAG-IgA, and 63% for AG-IgG; the sensitivities of anti-tTG were 90, 84, 84, andud75% when measured with Eurospital, Medipan, Inova, and Arnika kits respectively. udThe new AtTG seems to be accurate enough to be proposed as a noninvasiveuddiagnostic tool for CD diagnosis; the 4 kits analyzed showed similar diagnosticudefficacy.