NUTRIENT MEDIA FOR OBTAINING A PURE CULTURE OF FUNGI OF THE GENUS Pleurotus in vitro

摘要

The aim of the study was modification of the nutrient medium for basidiomycetes of the genus Pleurotus and its linear velocity of the growth determination. Biotechnology (obtaining and subcultivation of the strain NC-35 in vitro) and statistical methods were used. It was found that previously known media do not provide sufficient speed of mycelium growth and necessary biomass yield. There were investigated the new components of nutrient medium for greater biomass of pure culture of higher basidiomycetes of the genus Pleurotus obtaining. The nutrient medium for pure cultures of higher basidiomycetes, which contains agar-agar and water, additionally included the oat grain and oak bark decoctions in the following ratio per 1 l: agar-agar 15 g, oat grain decoction 600 ml, oak bark decoction 250 ml, distilled water — up to 1 l. The results of the linear growth rate analysis of Pleurotus ostreatus on the test and the control nutrient media, the calculation of the correlation coefficient indicate that for isolation and cultivation of oyster mushroom strains the most suitable medium is medium with the content of oat grain and oak bark decoctions. The obtained results will be used in the future for pure cultures of fungi of the genus Pleurotus obtaining in vitro.