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A NanoBRET-based binding assay for Smoothened allows real time analysis of small-molecule ligand binding and distinction of two separate ligand binding sites for BODIPY-cyclopamine

机译：用于平滑的基于纳米筋的结合测定允许实时分析小分子配体结合和不同的双链 - 环氨基的单独配体结合位点的区别

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Paweł Kozielewicz; Carl-Fredrik Bowin; Gunnar Schulte;
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年度 2019
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1. A NanoBRET-Based Binding Assay for Smoothened Allows Real-time Analysis of Ligand Binding and Distinction of Two Binding Sites for BODIPY-cyclopamine [J] . Kozielewicz Pawel, Bowin Carl-Fredrik, Turku Ainoleena, Molecular pharmacology. . 2020,第1期

机译：用于平滑的基于纳米筋的结合测定允许对BODIPY-Cyclopamine的两个结合位点进行实时分析
2. A systematic analysis of the effect of small-molecule binding on protein flexibility of the ligand-binding sites [J] . Yang CY, Wang RX, Wang SM Journal of Medicinal Chemistry . 2005,第18期

机译：系统分析小分子结合对配体结合位点蛋白质柔性的影响
3. BRET-BASED BINDING ASSAYS: PYRYLIUM-LABELED HISTAMINE RECEPTOR LIGANDS ALLOW FOR THE DETERMINATION OF BINDING CONSTANTS AT EQUILIBRIUM AND IN REAL TIME [J] . Graetz L., Littmann T., Bartole E., Inflammation research: Official journal of the European Histamine Research Society . 2019,第Suppla1期

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4. Optical fiber immunosensor for the real-time analysis of ligand-receptor binding kinetics [C] . Hua Lu, Southeast Univ., Nanjing, 1999 International Conference on Biomedical Optics . 1999

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5. Molecular interactions of adipocyte fatty acid-binding protein with activating and non-activating ligands: Protein oligomerization and ligand binding sites [D] . Rizk, Samar Helmy. 2013

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6. Quantitative Characterization of E-selectin Interaction with Native CD44 and P-selectin Glycoprotein Ligand-1 (PSGL-1) Using a Real Time Immunoprecipitation-based Binding Assay [O] . Dina B. AbuSamra, Alia Al-Kilani, Samir M. Hamdan, 2015

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7. A Systematic Analysis of the Effect of Small-Molecule Binding on Protein Flexibility of the Ligand-Binding Sites [O] . -1

机译：小分子结合对配体结合位点的蛋白质柔性作用的系统分析

A NanoBRET-based binding assay for Smoothened allows real time analysis of small-molecule ligand binding and distinction of two separate ligand binding sites for BODIPY-cyclopamine

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