A method for the separation of glycine-conjugated bile acids as a group from taurine-conjugated bile acids

摘要

A thin-layer chromatographic method for bile acids has been described which separates glycine conjugates, taurine conjugates, and free bile acids into three separate bands. The solvent system used for this purpose is ethanol-isopropyl alcohol-isooctane-ethyl acetate (25:10:10:10). This method is time saving and very simple for quantitative estimation of the glycine:taurine ratio in biological specimens.