This investigation was prompted by the findings that (1) dibucaine-resistant homozygotes and heterozygotes for plasmacholinesterase also exhibit resistance to fluoride inhibition, (2) the differentiation of dibucaine-resistant from the fluoride-resistant genotypes is ambiguous with the method of Harris and Whittaker, (3) the plasmacholinesterase inhibition by Na fluoride (FN) is markedly influenced by the temperature. Therefore, we modified their method by increasing (1) the temperature of the reaction from 25C to 37C and (2) the concentration of Na fluoride from 5.0×10 −5 m to 2.5×10 −4 m . With this method, genetically normal individuals have a mean FN± sd =77.0±3.22 while atypical dibucaine-resistant homozygotes have a mean FN± sd =43.0±10.0 and atypical dibucaine-resistant heterozygotes 67.0±5.37. Since a linear correlation was observed between DN and FN by our new method, a fluoride number 2 sd lower than the predicted FN from the DN can distinctly identify the fluoride-resistant plasmacholinesterase genotype E 1 f .
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机译:通过该研究结果提示调查(1)抗性纯合蛋白酶和杂种蛋白酶蛋白酶蛋白酶的结果也表现出对氟化物抑制的耐药性,(2)耐氟抗性基因型的Dibucaine抗性的分化与哈里斯和惠特克的方法模糊不清(3)(3)通过Na氟化物(Fn)的血管素酸酶抑制明显受温度的影响。因此,我们通过增加(1)从25℃至37℃的反应温度和(2)从5.0×10-5μm至2.5×10 -4m的浓度的反应的温度进行修饰(1)。通过这种方法,遗传正常个体具有平均值的Fn±Sd = 77.0±3.22,而非典型抗纤维酸纯合蛋白具有平均值的Fn±Sd = 43.0±10.0和非典型二匹替氏杂杂合子67.0±5.37。由于通过我们的新方法在DN和Fn之间观察到线性相关性,因此来自DN的预测的FN低的氟化物数2SD可以明显地鉴定氟化物抗性血管活性酶酶基因型E 1 F.
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