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In Vitro Antibacterial Activity of Eurycoma Longifolia Jack (Tongkat Ali) Root Extract

机译:毛杜鹃根提取物的体外抗菌活性。

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摘要

Introduction: Currently, researchers are aiming to explore herbal plants to replace synthetic drugs because herbal plants contain high active compounds and fewer side effects. Our study was done to determine the antibacterial activity of Eurycoma longifolia Jack (E. longifolia) root using ethanol based extract. Methods: Five types of pathogenic bacterial strains were used; Gram-positive (Staphylococcus aureus and Bacillus cereus) and Gram-negative (Escherichia coli, Salmonella typhi and Pseudomonas aeruginosa). Disc diffusion assay and Minimum Inhibitory Concentration (MIC) tests were used to determine the inhibition zone and turbidity of suspension which reflects the antibacterial activity of the extract. Results: The ethanolic extract of E. longifolia Jack root extract showed positive results against Gram-positive bacteria (S. aureus and B. cereus) and Gramnegative (S. typhi). B.cereus and S.typhi showed inhibition zone values of 11.76mm and 14.33mm at the extract concentration of 150mg/ml that were higher than the positive control values (9.00, 12.67mm) respectively. However, E. coli and P. aeruginosa did not show any inhibition by the ethanol-based extract. Conclusion: From the results we can conclude that E.Longifolia root extract possesses antibacterial activity that can be further explored to produce new medicinal products.
机译:简介:目前,研究人员旨在探索草药植物来替代合成药物,因为草药植物含有高活性化合物且副作用较少。我们的研究是通过使用乙醇基提取物来确定长叶杜仲(Eurycoma longifolia Jack)(E. longifolia)根的抗菌活性。方法:使用五种致病性细菌菌株。革兰氏阳性(金黄色葡萄球菌和蜡状芽孢杆菌)和革兰氏阴性(大肠杆菌,鼠伤寒沙门氏菌和铜绿假单胞菌)。椎间盘扩散测定和最小抑菌浓度(MIC)测试用于确定悬浮液的抑菌圈和浊度,从而反映提取物的抗菌活性。结果:E。longifolia Jack根提取物的乙醇提取物对革兰氏阳性菌(金黄色葡萄球菌和蜡状芽孢杆菌)和革兰氏阴性菌(伤寒沙门氏菌)显示出阳性结果。蜡状芽孢杆菌和鼠伤寒沙门氏菌在提取物浓度为150mg / ml时显示的抑制区值为11.76mm和14.33mm,分别高于阳性对照值(9.00、12.67mm)。然而,基于乙醇的提取物对大肠杆菌和铜绿假单胞菌没有显示任何抑制作用。结论:从结果中我们可以得出结论,龙脑叶根提取物具有抗菌活性,可以进一步探索以生产新的药用产品。

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