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Creation of a fully active, cytosolic form of human type I 3beta-hydroxysteroid dehydrogenase/isomerase by the deletion of a membrane-spanning domain

机译：通过缺失薄膜 - 跨越结构域创造一种完全活跃的，细胞溶质形式的人类I型3beta-羟类脱氢酶/异构酶

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JL Thomas; BW Evans; G Blanco; JI Mason; RC Strickler;
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年度 1999
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1. Rational proteomics V: structure-based mutagenesis has revealed key residues responsible for substrate recognition and catalysis by the dehydrogenase and isomerase activities in human 3beta-hydroxysteroid dehydrogenase/isomerase type 1. [J] . Pletnev VZ, Thomas JL, Rhaney FL, The Journal of Steroid Biochemistry and Molecular Biology . 2006,第1期

机译：理性蛋白质组学V：基于结构的诱变揭示了人类3β-羟基类固醇脱氢酶/异构酶1型中的脱氢酶和异构酶活性导致关键底物识别和催化的关键残基。
2. Site-directed mutagenesis identifies amino acid residues associated with the dehydrogenase and isomerase activities of human type I (placental) 3beta-hydroxysteroid dehydrogenase/isomerase. [J] . Thomas JL, Evans BW, Blanco G, The Journal of Steroid Biochemistry and Molecular Biology . 1998,第5a6期

机译：定点诱变确定与人类I型（胎盘）3β-羟基类固醇脱氢酶/异构酶的脱氢酶和异构酶活性相关的氨基酸残基。
3. Affinity radiolabeling identifies peptides associated with the isomerase activity of human type I (placental) 3beta-hydroxysteroid dehydrogenase/isomerase [J] . Thomas JL, Evans BW, Strickler RC Biochemistry . 1997,第29期

机译：亲和放射性标记可识别与人类I型（胎盘）3β-羟基类固醇脱氢酶/异构酶的异构酶活性相关的肽
4. Cytosolic isoform-like structure of domain II is important for the stability of ascorbate peroxidase of Galdieria partita under ascorbate-depleted conditions. [C] . S Kitajima, M Ueda, S Sano, International Congress on Photosynthesis . 2001

机译：结构域II的细胞溶质同种型结构对于Galdieria Partita的抗坏血酸过氧化物在抗坏血酸耗尽条件下是重要的。
5. INTRACELLULAR LOCALIZATION AND PROPERTIES OF 3BETA-HYDROXYSTEROID DEHYDROGENASE/ISOMERASE IN THE ADRENAL CORTEX [D] . CHAPMAN, JOHN CHAUNCEY. 1980

机译：肾上腺皮质中3BET-羟基类固醇脱氢酶/异构酶的细胞内定位和性质
6. RATIONAL PROTEOMICS V: STRUCTURE-BASED MUTAGENESIS HAS REVEALED KEY RESIDUES RESPONSIBLE FOR SUBSTRATE RECOGNITION AND CATALYSIS BY THE DEHYDROGENASE AND ISOMERASE ACTIVITIES IN HUMAN 3β-HYDROXYSTEROID DEHYDROGENASE/ISOMERASE TYPE 1 [O] . Vladimir Z. Pletnev, James L. Thomas, Felicia L. Rhaney, -1

机译：理性蛋白质组学V：基于结构的诱变已揭示关键残基可通过人3β-羟基甾体脱氢酶/异构酶类型中的脱氢酶和异构酶活性对基质识别和催化进行基质识别和催化。
7. The engineered, cytosolic form of human type I 3beta-hydroxysteroid dehydrogenase/isomerase: purification, characterization and crystallization [O] . JL Thomas, JI Mason, G Blanco, 2001

机译：人类I型3β-羟类脱氢酶/异构酶的工程化，细胞溶质形式：纯化，表征和结晶

Creation of a fully active, cytosolic form of human type I 3beta-hydroxysteroid dehydrogenase/isomerase by the deletion of a membrane-spanning domain

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