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Cas9-triggered chain ablation of cas9 as a gene drive brake

机译:Cas9触发的cas9链消融作为基因驱动制动器

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摘要

With the advent of clustered, regularly interspaced, short palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) technology, researchers can construct gene drives that can bias the inheritance of edited alleles to alter entire populations. As demonstrated with the mutagenic chain reaction in Drosophila4, the CRISPR-Cas9 system can propagate genomic modification together with the genome-editing machinery itself. Although gene drives might have the potential to control insect-borne diseases and agricultural pests, substantial concerns have been raised over unanticipated ecological consequences as a result of drive use. Here we report the development of a potential Cas9-based gene drive 'brake' that remains inert in a wild-type genome but is activated by Cas9 to both cleave the genomic cas9 sequence and to convert an incoming cas9 allele into a brake. This means that the propagation of the brake is favored in a cas9-carrying population.
机译:随着簇状,规则间隔,短回文重复序列(CRISPR)– CRISPR相关蛋白9(Cas9)技术的出现,研究人员可以构建基因驱动器,从而使编辑的等位基因的遗传偏向改变整个种群。正如果蝇4中的诱变链反应所证明的那样,CRISPR-Cas9系统可以与基因组编辑机制本身一起传播基因组修饰。尽管基因驱动器可能具有控制昆虫传播的疾病和农业害虫的潜力,但人们对驱动器使用带来的意想不到的生态后果提出了很大的关注。在这里,我们报告了一个潜在的基于Cas9的基因驱动“制动器”的开发,该制动器在野生型基因组中保持惰性,但是被Cas9激活以裂解基因组cas9序列并将传入的cas9等位基因转化为制动器。这意味着,在带有cas9的人群中,制动器的传播是有利的。

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