首页> 外文OA文献 >Genetics of the serine cycle in Methylobacterium extorquens AM1: identification, sequence, and mutation of three new genes involved in C1 assimilation, orf4, mtkA, and mtkB
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Genetics of the serine cycle in Methylobacterium extorquens AM1: identification, sequence, and mutation of three new genes involved in C1 assimilation, orf4, mtkA, and mtkB

机译:甲基芽胞杆菌AM1丝氨酸循环的遗传学:鉴定,序列和突变的三个新的基因参与C1同化,orf4,mtkA和mtkB

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摘要

In a recent paper we reported the sequence of the beginning of a serine cycle gene cluster on the Methylobacterium extorquens AM1 chromosome, containing the genes encoding serine glyoxylate aminotransferase (sgaA), hydroxypyruvate reductase (hprA), and 5,10-methylenetetrahydrofolate dehydrogenase (mtdA) (L. V. Chistoserdova and M. E. Lidstrom J. Bacteriol. 176:1957-1968, 1994). Here we present the sequence of the adjacent downstream region containing three full and one partial open reading frames. The first of the full open reading frames (orf4) remains unidentified, while the other two (mtkA and mtkB) code for the two subunits of malate thiokinase, and the fourth, a partial open reading frame (ppcA), apparently encodes phosphoenolpyruvate carboxylase. Mutants containing insertion mutations in orf4, mtdA, and mtdB all were unable to grow on C1 compounds, showing that these three newly identified genes are indispensable for the operation of the serine cycle. Mutants in orf4 were also unable to grow on C_2 compounds, but growth was restored by glyoxylate, suggesting that orf4 might be required for the conversion of acetyl coenzyme A to glyoxylate.
机译:在最近的一篇论文中,我们报道了甲基芽胞杆菌AM1染色体上丝氨酸循环基因簇的开始序列,该序列包含编​​码丝氨酸乙醛酸氨基转移酶(sgaA),羟基丙酮酸还原酶(hprA)和5,10-亚甲基四氢叶酸脱氢酶(mtdA)的基因。 (LV Chistoserdova和ME Lidstrom J.Bacteriol.176:1957-1968,1994)。在这里,我们介绍了包含三个完整和一个部分开放阅读框的相邻下游区域的序列。完全开放阅读框的第一个(orf4)尚未确定,而其他两个(mtkA和mtkB)编码苹果酸硫激酶的两个亚基,第四个,部分开放阅读框(ppcA),显然编码磷酸烯醇丙酮酸羧化酶。在orf4,mtdA和mtdB中包含插入突变的突变体均无法在C1化合物上生长,这表明这三个新近鉴定的基因对于丝氨酸循环的运作是必不可少的。 orf4中的突变体也无法在C_2化合物上生长,但是乙醛酸可恢复生长,这表明orf4可能需要将乙酰辅酶A转化为乙醛酸。

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