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Synthesis of Bacterial Flagella: Chromosomal Synchrony and Flagella Synthesis

机译:细菌鞭毛的合成:染色体同步与鞭毛的合成

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摘要

Synchronous cultures of Bacillus subtilis 168 M were obtained from light-density spores germinated at 46 C and grown at 37 C. This procedure synchronizes both cell division and chromosome replication. The chromosome synchrony was demonstrated by using transformation to measure changes in marker frequency during the cell cycle. The synthesis of two enzymes and of bacterial flagellar protein was also followed. All of the proteins were found to be synthesized continuously with an abrupt doubling in the rate of synthesis at a specific time in the cell cycle. The time at which the doubling occurred for each enzyme corresponded to the time at which the structural gene for the enzyme was replicated. The doubling of the rate of flagella synthesis corresponded to the time of replication of the hisA1 gene. We conclude that the genetic locus for the factors involved in the rate-limiting steps in flagella synthesis are located on the genetic map near the hisA1 locus.
机译:枯草芽孢杆菌168 M的同步培养物是从在46 C萌发并在37 C生长的光密度孢子获得的。此过程可同步细胞分裂和染色体复制。通过使用转化来测量细胞周期中标志物频率的变化,证明了染色体的同步性。还跟踪了两种酶和细菌鞭毛蛋白的合成。发现所有蛋白质都是连续合成的,在细胞周期的特定时间合成速率突然翻倍。每种酶发生倍增的时间对应于该酶的结构基因被复制的时间。鞭毛合成速率的倍增对应于hisA1基因的复制时间。我们得出结论,鞭毛合成中限速步骤所涉及因素的遗传基因座位于hisA1基因座附近的遗传图谱上。

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  • 作者单位
  • 年度 1969
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  • 原文格式 PDF
  • 正文语种 {"code":"en","name":"English","id":9}
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