We report the implementation of an on-chip microscope system, termed fluorescence optofluidic microscope (FOFM), which is capable of fluorescence microscopy imaging of samples in fluid media. The FOFM employs an array of Fresnel zone plates (FZP) to generate an array of focused light spots within a microfluidic channel. As a sample flows through the channel and across the array of focusedudlight spots, the fluorescence emissions are collected by a filter-coated CMOS sensor, which serves as the channel’s floor. The collected data can then be processed to render fluorescence microscopy images at a resolution determined by the focused light spot size (experimentally measured as 0.65 mm FWHM). In our experiments, our established resolution was 1.0 mm due to Nyquist criterion consideration. As a demonstration, we show that such a system can be used to image the cell nuclei stained by Acridine Orange and cytoplasm labeled by Qtracker.
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机译：我们报告了称为荧光光流体显微镜（FOFM）的片上显微镜系统的实现，该系统能够对流体介质中的样品进行荧光显微镜成像。 FOFM使用菲涅耳波带片（FZP）阵列在微流体通道内生成聚焦光斑阵列。当样本流过通道并穿过聚焦的 udlight点阵列时，荧光发射被用作通道底部的滤膜涂层CMOS传感器收集。然后可以对收集到的数据进行处理，以提供由聚焦光斑尺寸（实验测量为0.65 mm FWHM）确定的分辨率的荧光显微镜图像。在我们的实验中，由于奈奎斯特准则的考虑，我们确定的分辨率为1.0 mm。作为演示，我们表明该系统可用于对be啶橙染色的细胞核和Qtracker标记的细胞质进行成像。
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