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Efficient CRISPR/Cas9 mediated Pooled-sgRNAs assembly accelerates targeting multiple genes related to male sterility in cotton

机译：高效的CRISPR / CAS9介导的汇集-SGRNAS组装加速靶向棉花中雄性不育的多种基因

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Mohamed Ramadan; Muna Alariqi; Yizan Ma; Yanlong Li; Zhenping Liu; Rui Zhang; Shuangxia Jin; Ling Min; Xianlong Zhang;
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1. Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation [J] . Wang Zhi-Ping, Xing Hui-Li, Dong Li, Genome Biology . 2015,第7期

机译：卵细胞特异的启动子控制的CRISPR / Cas9可在单个世代中有效产生拟南芥中多个靶基因的纯合突变体
2. Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation [J] . Zhi-Ping Wang, Hui-Li Xing, Li Dong, Genome Biology . 2015,第1期

机译：卵细胞特异的启动子控制的CRISPR / Cas9可在单代中高效产生拟南芥中多个靶基因的纯合突变体
3. Efficient and transgene-free gene targeting using Agrobacterium-mediated delivery of the CRISPR/Cas9 system in tomato [J] . Danilo Benoit, Perrot Laura, Mara Kostlend, Plant Cell Reports . 2019,第4期

机译：使用Agrobacterium介导的Trypr / Cas9系统递送的高效和转基因的基因靶向番茄中的CRISPR / CAS9系统
4. ACCELERATED HOMOLOGY-DIRECTED TARGETED INTEGRATION OF TRANSGENES IN CHO CELLS VIA CRISPR/CAS9 AND FLUORESCENT ENRICHMENT [C] . Jae Seong Lee, Lise Marie Grav, Gyun Min Lee, Cell culture engineering XV . 2016

机译：通过CRISPR / CAS9和荧光富集加速同源性定向的CHO细胞中转基因的定向整合
5. Advancements in the Understanding and Treatment of Neurodegenerative Diseases Accelerated by CRISPR/Cas9 [D] . Cassidy, Jesse 2017

机译：CRISPR / Cas9促进神经退行性疾病认识和治疗的进展
6. Efficient CRISPR/Cas9 mediated Pooled-sgRNAs assembly accelerates targeting multiple genes related to male sterility in cotton [O] . Mohamed Ramadan, Muna Alariqi, Yizan Ma, 2021

机译：高效的CRISPR / CAS9介导的汇集-SGRNAS组装加速靶向棉花中雄性不育的多种基因
7. The cloning and CRISPR/Cas9‐mediated mutagenesis of a male sterility gene MS1 of soybean [O] . Bingjun Jiang, Li Chen, Chunyan Yang, 2021

机译：克隆和CRAP / CAS9介导的大豆雄性不育基因MS1的诱变诱变

Efficient CRISPR/Cas9 mediated Pooled-sgRNAs assembly accelerates targeting multiple genes related to male sterility in cotton

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