ABSTRAK Protease alkaline and keratinase are a group of protease enzym which have important value in detergen industry and skin tannery. Brevibacillus agri A-03 is thermophilic bacteria isolate that comes from Ambayan Sumatera Barat hot spring and has the ability to produce protease and keratinase. The purpose of this research is to get protease alkaline and thermostable keratinase from Brevibacilus agri-A03. thermostable enzym is produced from enzym production enzym that contains kasein and keratin at various medium pH, inoculum incubation temperature and medium type. Enzym activity is measured by modified Walker methode, protein content is measured by Lowry methode. Protease alkaline is produced at exponential phase, maximum at 18th hours of incubation and keratinase is produced at stationer phase, maximum at 22nd hours. Both enzym is produced optimically at medium pH condition 9.0; incubation temperature 55°C, inoculum 5% by using modified Johnvesly and Naik medium with each protease and keratinase specific activity 1.927 and 1.047 U/mg Keywords: Protease alkaline, Keratinase, Thermofilic, Brevibacillus agri A-03
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机译：Abstrak蛋白酶碱性和角蛋白酶是一组蛋白酶酶，其在污水工业和皮肤制革器中具有重要价值。 Brevibacillus Agri A-03是来自Ammayan Sumatera Barat温泉的嗜热细菌孤立物，具有产生蛋白酶和角蛋白酶的能力。本研究的目的是从Brevibacilus Agri-A03获得蛋白酶碱性和热稳定角蛋白酶。热稳定酶由酶生产酶产生，该酶在各种培养基，接种物培养温度和中等型磷酸酯和角蛋白中产生。酶活性通过改性步行者甲基测量，蛋白质含量通过Lowry Methode测量。蛋白酶碱性在指数阶段产生，最大在第18小时的孵育和角蛋白酶在寄生阶段产生，最大在22nd小时。两种酶在培养基条件9.0时良好地生产;孵育温度55°C，通过使用改性的Johvesly和Naik培养基和角蛋白酶和角蛋白酶特异性活性为1.927和1.047 U / Mg关键词：蛋白酶碱性，角蛋白酶，热硅，Brevibacillus Agri A-03
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