The high incidence of bacterial vaginosis recurrence is common after treatment with an antibiotic agent and suggests the need for new treatments to prevent this. We conducted a randomized trial to evaluate the ability of maltose gel to treat bacterial vaginosis. Eighteen female rhesus macaques were randomly assigned, in a 2:1 ratio, to receive maltose gel or placebo gel by syringe to the fornix of the vagina for five consecutive days. We used 16S rRNA sequencing data from 70 swab samples of vaginal secretions in two groups in total on days 0, 3, and 5 after medication initiation and days 3 and 5 after medication withdrawal for the study of microbiome composition. We found that, in the placebo control group, there was no significant change in the composition and abundance of vaginal microbiota during the follow-up period. In the maltose gel test group, the abundance of Lactobacillus in the vagina microbiota increased gradually with the prolongation of the treatment time on Days 3 and 5 (ANOVA p = 6.99e−5 < 0.01) but began to decrease after the withdrawal of maltose gel, which was different from that of the control group. Correspondingly, the diversity and abundance of BV-related bacteria, Fusobacterium, Parvimonas, Mobiluncus, Campylobacter, Prevotella, and Sneathia, decreased on Day 0 to Day 5 of medication and increased after drug withdrawal in the maltose gel test group. The study confirms that maltose gel can facilitate the proliferation of Lactobacillus and promote the transition of the vaginal microbiota from BV-related bacteria dominant to Lactobacillus dominant in the rhesus macaque.
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机译:在用抗生素药物处理后,细菌性阴道症复发的高发病率是常见的,并表明需要新的治疗方法以防止这种情况。我们进行了一项随机试验,以评估麦芽糖凝胶治疗细菌性阴道病的能力。随机分配18只女性恒河猴,在2:1的比例中,通过注射器将麦芽糖凝胶或安慰剂凝胶接收到阴道的末端连续五天。我们在两组中使用了来自70次的阴道分泌物的rRNA测序数据,总共0,3和5天,在药物启动后和第3天和第3天和第5天,用于研究微生物组组合物的研究。我们发现,在安慰剂对照组中,在随访期间阴道微生物群的组成和丰度没有显着变化。在麦芽糖凝胶试验组中,阴道微生物酵母的丰度随着第3天和第5天(ANOVA P = 6.99E-5 <0.01)延长的治疗时间逐渐增加(ANOVA P = 6.99E-5 <0.01),但在麦芽糖凝胶撤离后开始减少,与对照组不同。相应地,BV相关细菌,Fusobacterium,Parvimonas,Mobiluncus,Campyloback action,Phivotella和Sneateia的多样性和丰度,每天减少到药物的第0天,并在麦芽糖凝胶试验组中取出后增加。该研究证实,麦芽糖凝胶可以促进乳杆菌的增殖,并促进阴道微生物群从与羊颈猕猴的乳酸杆菌显着显着的BV相关细菌的转变。
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