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Functional Heterologous Expression of Mature Lipase LipA from Pseudomonas aeruginosa PSA01 in Escherichia coli SHuffle and BL21 (DE3): Effect of the Expression Host on Thermal Stability and Solvent Tolerance of the Enzyme Produced

机译：从大肠杆菌Coli Shuffle和BL21（DE3）中PSEUDOMONAS铜绿假单胞菌PSA01的功能异源表达：表达宿主对所产生的酶的热稳定性和溶剂耐受的影响

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Ingrid Yamile Pulido; Erlide Prieto; Gilles Paul Pieffet; Lina Méndez; Carlos A. Jiménez-Junca;
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1. Enhancing Functional Expression of Codon-Optimized Heterologous Enzymes in Escherichia coli BL21(DE3) by Selective Introduction of Synonymous Rare Codons [J] . Zhong Chao, Wei Ping, Zhang Yi-Heng Percival Biotechnology and Bioengineering . 2017,第5期

机译：通过选择性引入同义稀有密码子来增强Codon优化异源酶的功能性表达
2. Enhancing Functional Expression of Codon-Optimized Heterologous Enzymes in Escherichia coli BL21(DE3) by Selective Introduction of Synonymous Rare Codons [J] . Zhong Chao, Wei Ping, Zhang Yi-Heng Percival Biotechnology and Bioengineering . 2017,第5期

机译：通过选择性引入同义稀有密码子来增强CONCHERICA COLI BL21（DE3）中的密码子优化异源酶的功能表达
3. Cloning, expression and characterization of aeruginosa EGYII L-Asparaginase from Pseudomonas aeruginosa strain EGYII DSM 101801 in E.coli BL21(DE3) pLysS [J] . El-Sharkawy Amany S., Farag Aida M., Embaby Amira M., Journal of molecular catalysis, B. Enzymatic . 2016,第Null期

机译：铜绿假单胞菌EGYII DSM 101801中铜绿假单胞菌EGYII L-天冬酰胺酶在大肠杆菌BL21（DE3）pLysS中的克隆，表达及鉴定
4. EFFECT OF PH AND AERATION ON PLASMID STABILITY AND PHYTASE EXPRESSION IN Escherichia coli BL21(DE3) DURING BATCH CULTIVATIONS IN SEMI-SCALE BIOREACTOR [C] . Othman N.Z, Ramli.S, Masri J.H, AIChE annual meeting . 2012

机译：半批量生物反应器分批培养过程中PH和曝气对大肠杆菌BL21（DE3）质体稳定性和植酸酶表达的影响
5. Identification of factors limiting heterologous lipase expression in the cytoplasm and the periplasm as well as display on cell surface of Escherichia coli. [D] . Xu, Yali. 2008

机译：鉴定限制异源脂肪酶在细胞质和周质中表达以及在大肠杆菌细胞表面上展示的因素。
6. Functional Heterologous Expression of Mature Lipase LipA from Pseudomonas aeruginosa PSA01 in Escherichia coli SHuffle and BL21 (DE3): Effect of the Expression Host on Thermal Stability and Solvent Tolerance of the Enzyme Produced [O] . Ingrid Yamile Pulido, Erlide Prieto, Gilles Paul Pieffet, 2020

机译：铜绿假单胞菌PSA01中成熟脂肪酶LipA在大肠杆菌SHuffle和BL21（DE3）中的功能异源表达：表达宿主对所产生酶的热稳定性和溶剂耐受性的影响
7. Table 1: Purification of T1 lipase from a newly constructed GSTm-T1 lipase from Escherichia coli BL21(DE3)pLysS as an expression system. [O] . -1

机译：表1：从大肠杆菌BL21（DE3）帘布层作为表达系统，从新构建的GSTM-T1脂肪酶纯化T1脂肪酶。

Functional Heterologous Expression of Mature Lipase LipA from Pseudomonas aeruginosa PSA01 in Escherichia coli SHuffle and BL21 (DE3): Effect of the Expression Host on Thermal Stability and Solvent Tolerance of the Enzyme Produced

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