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Question 5 Given a fragment of mRNA sequence, how would one find where that piece of DNA mapped in the human genome? Once its position has been determined, how would one find alternatively spliced transcripts?

机译：问题5给定mRNA序列的片段，如何找到在人类基因组中映射的那段DNA的地方？一旦确定其位置，人们将如何发现拼接转录物？

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1. Identification and characterization of a novel cyclic nucleotide phosphodiesterase gene (PDE9A) that maps to 21q22.3: alternative splicing of mRNA transcripts, genomic structure and sequence. [J] . Guipponi M, Scott HS, Kudoh J, Human Genetics . 1998,第4期

机译：鉴定和表征映射到21q22.3的新型环状核苷酸磷酸二酯酶基因（PDE9A）：mRNA转录本，基因组结构和序列的可变剪接。
2. Genome-wide analysis of alternative pre-mRNA splicing in Arabidopsis thaliana based on full-length cDNA sequences [J] . Iida K, Seki M, Sakurai T, Nucleic Acids Research . 2004,第17期

机译：基于全长cDNA序列的拟南芥备选mRNA剪接的全基因组分析
3. Genome-wide analysis of alternative pre-mRNA splicing in Arabidopsis thaliana based on full-length cDNA sequences [J] . Akihiko Konagaya, Kazuo Shinozaki, Kei Iida, Nucleic acids research . 2004,第17期

机译：基于全长cDNA序列的拟南芥备选mRNA剪接的全基因组分析
4. Widespread predicted nonsense-mediated mRNA decay of alternatively-spliced transcripts of human normal and disease genes [C] . Richard E. Green, Benjamin P. Lewis, R. Tyler Hillman, International Conference on Intelligent Systems for Molecular biology . 2003

机译：普遍预测的无意义介导的mRNA衰减的人正常和疾病基因的替代剪接转录物
5. The patchy human genome: DNA double-strand breaks in human cells can be repaired by the capture of other DNA fragments. [D] . Little, Kevin C. E. 2005

机译：斑驳的人类基因组：人类细胞中的DNA双链断裂可以通过捕获其他DNA片段来修复。
6. Genome-wide analysis of alternative pre-mRNA splicing in Arabidopsis thaliana based on full-length cDNA sequences [O] . Kei Iida, Motoaki Seki, Tetsuya Sakurai, 2004

机译：基于全长cDNA序列的拟南芥中备选mRNA剪接的全基因组分析
7. Large-scale identification and characterization of alternative splicing variants of human gene transcripts using 56 419 completely sequenced and manually annotated full-length cDNAs [O] . Takeda J-I, Suzuki Y., Nakao M., 2006

机译：使用56 419个完全测序并人工注释的全长cDNA大规模鉴定和表征人类基因转录本的可变剪接变体

Question 5 Given a fragment of mRNA sequence, how would one find where that piece of DNA mapped in the human genome? Once its position has been determined, how would one find alternatively spliced transcripts?

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