Prevalence of Enterotoxin Genes and spa Genotypes of Methicillin-resistant Staphylococcus aureus from a Tertiary Care Hospital in China

摘要

Objectives: Methicillin-resistant Staphylococcus aureus (MRSA)is a major nosocomial pathogen that causes a variety ofinfections. MRSA has evolved resistance to multiple antibiotics.Genetic background and virulence differs in different geographicregions. The present study was aimed to investigate theprevalence of enterotoxin genes and spa genotypes of hospitalassociated methicillin-resistant Staphylococcus aureus (HAMRSA) isolated from a tertiary care hospital of Jiangsu province,China.Materials and Methods: HA-MRSA isolates from August 2013to April 2014 at a tertiary care hospital of China were collected.We investigated antimicrobial pattern, spa types, SCCmectypes and the presence of 14 virulence genes.Results: Eighty HA-MRSA isolates were collected. Results fromSCCmec typing revealed that 73.8% were type II; 13.8% weretype III; 12.5% were type V. There were 19 different spa types.Spa type t2460 was the most common (35.0%), followed byt002 (11.3%). CC5 was the predominant MLST CCs type (50%).The most frequent toxin genes were sea, seb, sed, sel, sen andseo (100.0%). None of the investigated isolates carried the secor tst.Conclusion: Genotypic and virulence evaluation of the isolatedHA-MRSA revealed that the isolates with CC5 and SCCmecII were the predominant type and highly homological. Thevirulence profiles mainly existed in the genes of sea, seb, sed,sel, sen, seo and ser. The prevalence of t2460 was an outbreakand the predominant spa type.