Label-Free Proteomic Analysis of Wheat Gluten Proteins and Their Immunoreactivity to ELISA Antibodies

摘要

ELISA (enzyme linked immunosorbent assay) methodology is currently the mainstay for gluten quantification. However, the lack of comparable measurements among commercial kits has caused a great deal of concern. Here, we studied the immunoreactivity of five commercial ELISA kits to wheat gluten fractionated by reversed phase high-performance liquid chromatography (RP-HPLC) and identified the proteins and peptides in the resulting fractions by mass spectrometry to understand the extent by which these may be contributing to the lack of comparability.