Wide-field multiphoton imaging through scattering media without correction

摘要

We demonstrate an approach for wide-field multiphoton imaging throughscattering media without any a priori knowledge or requirement to determine itsproperties. Temporal focusing is used to project light patterns in a Hadamardbasis through a turbid medium, and the sample fluorescence is collected backthrough the very same objective in a single-pixel imaging geometry. We imagemicroscopic fluorescent beads and human embryonic kidney cells over a field ofview of 90$imes$90 $mu$m$^2$ through various turbid media. This includes ascattering phantom, colon and brain tissue, up to 540 $mu$m in thickness. Theproposed method provides a step change for imaging as it obviates the need forany form of wavefront correction, any time-gated approach, or determination ofthe transmission matrix of the scattering medium.