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Comparative evaluation of two DNA isolation techniques for PCR-based diagnosis of gastrointestinal nematode infections in sheep

机译:两种DNA分离技术对绵羊胃肠道线虫感染的PCR基于DNA分离技术的对比评价

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摘要

The specific diagnosis of gastrointestinal parasite infections in livestock is central to their control. PCR assays have been developed for routine diagnosis and to overcome limitations of classical methods. Central to the performance of such assays is the effective isolation of the nucleic acids from samples and the elimination of components that are inhibitory to PCR. Here, we directly compared two techniques for the isolation of DNA from strongylid nematode eggs from faecal samples from sheep, and assessed their performance in relation to the sensitivity and specificity of PCR, time required for DNA isolation and ease of use. The results showed differences in the performance of the two isolation techniques, subsequently effecting the PCR results. The main differences related to the time required for DNA isolation, and the elimination of inhibitory substances from the DNA isolated by one technique but not the other.
机译:牲畜中胃肠道寄生虫感染的特异性诊断是对照的核心。已经开发了PCR测定用于常规诊断和克服古典方法的限制。这种测定的性能的核心是有效地分离来自样品的核酸和消除抑制PCR的组分。在这里,我们将两种技术与来自绵羊的粪便样品中的抗震线虫卵分离的两种技术与来自绵羊的粪便样品分离,并评估了它们与PCR的敏感性和特异性相关的性能,DNA分离所需的时间和易用性。结果显示出两种隔离技术的性能差异,随后效应PCR结果。与DNA分离所需的时间有关的主要差异,以及从一种技术中消除来自DNA的抑制物质,但不是另一个技术。

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