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SaCas9 Requires 5′-NNGRRT-3′ PAM for Sufficient Cleavage and Possesses Higher Cleavage Activity than SpCas9 or FnCpf1 in Human Cells

机译：SACAS9需要5'-NNGRRT-3'PAM足够的裂解，并且比人细胞中的SPCAS9或FNCPF1具有更高的切割活性

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Haihua Xie; Lianchao Tang; Xiubin He; Xiexie Liu; Chenchen Zhou; Junjie Liu; Xianglian Ge; Jin Li; Changbao Liu; Junzhao Zhao; Jia Qu; Feng Gu; Zongming Song;
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1. SaCas9 Requires 5′‐NNGRRT‐3′ PAM for Sufficient Cleavage and Possesses Higher Cleavage Activity than SpCas9 or FnCpf1 in Human Cells [J] . Xie Haihua, Tang Lianchao, He Xiubin, Biotechnology Journal: Healthcare,Nutrition,Technology . 2018,第3期

机译：SACAS9需要5'-NNGRRT-3'PAM用于足够的裂解，并且比人细胞中的SPCAS9或FNCPF1具有更高的切割活性
2. SaCas9 Requires 5′‐NNGRRT‐3′ PAM for Sufficient Cleavage and Possesses Higher Cleavage Activity than SpCas9 or FnCpf1 in Human Cells [J] . Xie Haihua, Tang Lianchao, He Xiubin, Biotechnology Journal: Healthcare,Nutrition,Technology . 2018,第3期

机译：SACAS9需要5'-NNGRRT-3'PAM足够的裂解，并且比人细胞中的SPCAS9或FNCPF1具有更高的切割活性
3. SaCas9 Requires 5-NNGRRT-3 PAM for Sufficient Cleavage and Possesses Higher Cleavage Activity than SpCas9 or FnCpf1 in Human Cells [J] . Xie Haihua, Tang Lianchao, He Xiubin, Biotechnology Journal: Healthcare,Nutrition,Technology . 2018,第4期

机译：SACAS9需要5-NNGRRT-3 PAM，足够的裂解，并且比人细胞中的SPCAS9或FNCPF1具有更高的切割活性
4. Are the mechanical field parameters sufficient to predict uniquely the failure due to the ductile or cleavage mechanisms? [C] . Andrzej Neimitz, Jaroslaw Galkiewicz European Conference on Fracture . 2019

机译：是否足以预测由于延性或裂解机制引起的唯一故障的机械场参数？
5. Prevention of Respiratory Syncytial Virus Attachment Protein Cleavage in Vero Cells Rescues Infectivity of Progeny Virions for Primary Human Airway Cultures. [D] . Corry, Jacqueline D. 2015

机译：预防Vero细胞中呼吸道合胞病毒附着蛋白的切割可拯救子代病毒对原代人气道培养物的感染性。
6. A ‘new lease of life’: FnCpf1 possesses DNA cleavage activity for genome editing in human cells [O] . Mengjun Tu, Li Lin, Yilu Cheng, 2017

机译：生命的新租赁：FnCpf1具有DNA裂解活性可在人类细胞中进行基因组编辑
7. Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants [O] . Eszter Tóth, Bernadett C Czene, Péter I Kulcsár, 2018

机译：MB-和FNCPF1核酸酶在哺乳动物细胞中活跃：四种野生型CPF1核酸酶的活性和PAM偏好以及其改变的PAM特异性变体
8. Detection of siRNA Mediated Target mRNA Cleavage Activities in Human Cells by a Novel Stem-Loop Array RT-PCR Analysis. [R] . Xu, K., Roth, J. A., Ji, L., 2016

机译：通过新型茎环阵列RT-pCR分析检测siRNa介导的人细胞靶mRNa切割活性。

SaCas9 Requires 5′-NNGRRT-3′ PAM for Sufficient Cleavage and Possesses Higher Cleavage Activity than SpCas9 or FnCpf1 in Human Cells

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