Detection of biomolecules using optoelectronic biosensor based on localized surface plasmon resonance. Nanoimprint lithography approach

摘要

In this work, we use a nanoimprint lithography fabrication technology toudcreate uniformly-oriented and homogenous noble metal nanoparticle arrays with wellcontrolledudsize, shape and spacing, which can be the basis platform for the developmentudof Localized Surface Plasmon Resonance (LSPR) sensors for biomolecular detection.udUsing this approach, we demonstrate proof-of-principle of an optical biosensor toudquantify biomolecular interactions in a real-time mode using a UV-visibleudspectrophotometer. The sensor shows concentration-dependent kinetics of surfaceudadsorption or binding of biomolecules and a capability to monitor antigen-antibodyudspecific reactions, which is demonstrated by the reaction between bovine serum albuminud(BSA) and anti-BSA immunoglobulin.