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Fabrication of Carbon Disulfide Added Colloidal Gold Colorimetric Sensor for the Rapid and On-Site Detection of Biogenic Amines

机译:碳二硫化碳的制备增加了胶体金色传感器,用于生物胺的快速和现场检测

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摘要

Meat is often wasted due to the perceived concerns of its shelf life and preservation. Specifically, in meat formation, biogenic amines (BAs) are the major agents to spoil them. Herein, we have developed a carbon disulfide (CS2) added colloidal gold nanoparticles-based colorimetric sensor for the rapid and on-site detection of biogenic amines. Transmission electron microscopy is used to observe the morphological changes in colloidal gold nanoparticles and aggregation behavior of CS2 added to the colloidal gold nanoparticles’ solution. Raman spectroscopic analysis is further used to characterize the peaks of CS2, Cad and CS2-Cad molecules. Absorption spectroscopy is used to estimate the colorimetric differences and diffuse reflectance spectra of the samples. The sensing analysis is performed systematically in the presence and absence of CS2. CS2 added colloidal gold nanoparticles colorimetric sensor detected the BAs with a limit of detection (LOD) value of 50.00 µM. Furthermore, the developed sensor has shown an LOD of 50.00 µM for the detection of multiple BAs at a single time. The observed differences in the colorimetric and absorption signals indicate that the structure of BAs is converted to the dithiocarbamate (DTC)-BA molecule, due to the chemical reactions between the amine groups of BAs and CS2. Significantly, the developed colorimetric sensor offers distinct features such as facile fabrication approach, on-site sensing strategy, rapid analysis, visual detection, cost-effective, possibility of mass production, availability to detect multiple BAs at a single time and appreciable sensitivity. The developed sensor can be effectively used as a promising and alternative on-site tool for the estimation of BAs.
机译:由于其保质期的担忧和保存的担忧,肉通常浪费。具体地,在肉形成中,生物胺(BAS)是破坏它们的主要药剂。在此,我们已经开发了一种基于胶体金纳米颗粒的比色传感器的碳二硫化物(CS2)进行了基于生物胺的快速和现场检测。透射电子显微镜用于观察在胶体金纳米颗粒的形态学变化,并加入到胶体金纳米颗粒溶液CS2的聚集行为。拉曼光谱分析还用于表征CS2,CAD和CS2-CAD分子的峰。吸收光谱法用于估计样品的比色差异和漫反射光谱。在存在和不存在CS2的情况下系统地进行感测分析。 CS2添加的胶体金纳米颗粒比色传感器检测到具有50.00μm的检测极限(LOD)值的基座。此外,开发的传感器已经显示为50.00μm的床位,用于在单个时间检测多个浅块。所观察到的比色和吸收信号的差异表明,由于碱和Cs2的胺基之间的化学反应,将BAS的结构转化为二硫代氨基甲酸(DTC)-BA分子。显着显着,展开的比色传感器提供了独特的特点,如Facile的制造方法,现场感测策略,快速分析,视觉检测,经济效益,批量生产的可能性,可在一次时间和可观的灵敏度检测多个BAS的可用性。开发的传感器可以有效地用作估计BAS的有希望和替代的现场工具。

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