Isolation of Nucleic Acids from Different Biological Objects with Silica—Magnetite Nanoparticles

摘要

Now, modified magnetic particles are widely used in different biological andudmedical applications (enzyme and protein immobilization, cells separationudand purification, MRI, targeted drug delivery, etc.). The aim of the presentudstudy is to reveal the ability of synthesized silica-modified magnetic particlesudto isolate DNA from the biological tissues in comparison with commonudmethod used the non-magnetic particles. Magnetite (Fe3O4) particles are preparedudvia co-precipitation of Fe+2 and Fe+3 with NH4OH in aqueous solution.udSilica—magnetite nanocomposites are prepared via tetraethoxysilane hydrolyzationudin alcohol—water—ammonia mixture. The average core size of synthesizedudmagnetic nanoparticles is about 15 nm (according to the TEM data).udApplication of these compounds for DNA isolation from different biologicaludobjects showed significant time-savings, overall higher yields, lower RNAudcontamination and better polymerase chain reaction (PCR) amplificationudcompared to commercial available silica non-magnetic particles (Promega).udHigh efficiency of nucleic-acid purification by silica—magnetite particles isudconfirmed in molecular assays with reverse transcriptase—polymerase chainudreaction (RT—PCR) assays of RNA- and DNA-virus diseases of plants, avian,udcattle and estimation of bacterial spectrum in dairy products (probiotics).