BRCA1 and MAD2 Are Coexpressed and Are Prognostic Indicators in Tubo-ovarian High-Grade Serous Carcinoma

摘要

Objectives: To investigate the relationship between BRCA1 and MAD2 protein expression, as determined by immunohistochemistry, and clinical outcomes in epithelial ovarian carcinoma (EOC). Methods: A tissue microarray (TMA) consisting of 94 formalin-fixed paraffin-embedded EOC with fully matched clinicopathological data were immunohistochemically stained with anti-BRCA1 and anti-MAD2 antibodies. The cores were scored in a semi-quantitative manner evaluating nuclear staining intensity and extent. Co-expression of BRCA1 and MAD2 was evaluated and patient survival analyses were undertaken. Results: Co-expression of BRCA1 and MAD2 was assessed in 94 EOC samples, and survival analysis was performed on 65 high grade serous carcinomas (HGSC). There was a significant positive correlation between BRCA1 and MAD2 expression in this patient cohort (p 0.0001). Both low BRCA1 and low MAD2 are independently associated with overall survival (OS) due to HGSC. Low co-expression of BRCA1 and MAD2 was also significantly associated with overall survival and was driven by BRCA1 expression. Conclusion: BRCA1 and MAD2 expression are strongly correlated in EOC but BRCA1 expression remains the stronger prognostic factor in HGSC.