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A manual method to obtain platelet rich plasma

机译:一种获取富含血小板血浆的手动方法

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摘要

OBJECTIVE:This study is to report a manual method to obtain platelet rich plasma (PRP).METHODS:For this study 61 ml of peripheral blood was obtained and submitted to centrifugation at 541g for 5 min. The centrifugation separates the blood into three components: red blood cells, buffy coat and platelet rich plasma. Blood and platelet rich plasma samples were sent to the Hospital's Laboratory and platelets and leukocytes were measured.RESULTS:A sample of 637 blood donors was evaluated. The platelet yield efficiency was 86.77% and the increase in platelet concentration factor was 2.89 times. The increase in leukocyte concentration factor was 1.97 times.CONCLUSION:The method described here produces leukocyte-rich and platelet-rich plasma with a high platelet and leukocyte increased factor.Level of Evidence IV, Controlled Laboratory Study.
机译:目的:本研究报告了一种获取富血小板血浆(PRP)的手动方法。方法:本研究获得了61 ml的外周血,并在541 g下离心5分钟。离心将血液分为三个部分:红细胞,血沉棕黄层和富含血小板的血浆。将血液和富含血小板的血浆样品送至医院的实验室,测量血小板和白细胞。结果:评估了637名献血者的样品。血小板产率为86.77%,血小板浓缩倍数增加2.89倍。结论:本文所述方法可产生富含白细胞和富含血小板的血浆,并具有较高的血小板和白细胞增加因子。证据水平IV,对照实验室研究。

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