PCR-SSCP and Hin6I PCR-RFLP detecting novel anonymous SNPs within ovine PROP1 gene (Brief report)

摘要

plays a direct or indirect key role in the ontogenesis of pituitarygonadotropes, as well as somatotropes, lactotropes and caudomedial thyrotropes (WU etal., 1998). Mutations of are responsible for deficiencies of POU1F1, GH,prolactin (PRL) and thyroid stimulating hormone (TSH) in Snell and Jackson dwarfmice and in man, as well as deficiencies of LH and FSH, suggesting that the gene is a potential candidate gene associating with production traits in animalbreeding and genetics. To date, no polymorphism of gene has been reported inruminant animals except bovine (PAN et al., 2007). So, the objective of this study isto investigate the polymorphism of all coding regions within ovine gene byPCR-SSCP, DNA sequencing and PCR-RFLP methods, which will possibly contributeto conducting association analysis and evaluating them as genetic markers in animalbreeding and genetics.