Molecular cloning, sequence characterization, and geneexpression profile of a novel water buffalo (Bubalusbubalis) gene: Na+, K+-ATPase β2-subunit (ATP1B2)

摘要

The Na, K-ATPase is a transmembrane carrier protein which plays an important role in Na-Ktransport and energy metabolism. The aim of this study was to obtain the full-length codingsequence (CDS) of water buffalo Na, K-ATPase β-subunit () using RT-PCR and toinvestigate the characterizations of its sequence and tissue expression patterns. Sequenceanalysis revealed that the CDS of water buffalo encodes an enzyme of 290 aminoacid residues with a deduced molecular weight of 33.39 KDa and a PI of 8.37. Water buffalo was presumed to have a signal peptide, a strong hydrophobic region and to exertits function in the plasma membrane with high reliability. In addition, water buffalo has a conserved Na, K-ATPase β domain which belongs to Na, K-ATPase superfamily. Thesequence of water buffalo gene shares 97.6, 97.4, 92.2, 93.2, 89.9, 93.2, 89.5 and 62.9 %identify with its homologous sequence of cattle, sheep, dog, human, mouse, baboon, ratand African clawed frog, respectively. Phylogenetic tree analysis based on the CDS of gene showed that water buffalo has a closer genetic relationship with cattle than with otherspecies. The gene was widely expressed in the tissues examined, being high in thepituitary gland and brain, moderate in the muscle, spleen, liver, mammary gland, kidneyand rumen, weak in the heart, small intestines and skin, and not expressed in the lung andadipose tissue. This study will establish a foundation for further insights into this novel waterbuffalo gene.