Cytogenetic and radiation hybrid mapping of the bovine interleukin 4 induced 1 (IL4I1) gene to cattle chromosome 18 (Brief Report)

摘要

The gene, also known as , encodes a lysosomal proteinin B cells with L-amino oxidase (EC 1.4.3.2) property and a high preference for aromaticamino acid substrates implicating a fundamental role in antigene processing andpresentation (MANSON et al. 2004). In human, has been assigned to chromosome19q13.3-19q13.4, a hot spot for susceptibility to several autoimmune diseases (CHAVANet al. 2002). Based on comparative genome data between human and cattle (GOLDAMMERet al. 2002), an assignment of to Bos taurus chromosome 18 (BTA18) has beenpredicted within a quantitative trait locus for somatic cell score (KÜHN et al. 2003). Thisprediction is supported by the putative bovine sequence for , LOC520692, which hasbeen assigned to BTA18 close to 56.1 megabases within the annotated bovine sequence(NCBI build Btau-4.0). Therefore, we started positional cloning of bovine , as acandidate gene related to adaptive immunity, i.e. during udder infections in cattle. Herewe present the physical assignment of the bovine gene by fluorescence in situhybridization (FISH) and radiation hybrid (RH) mapping.