Small cardamom (Elettaria cardamomum (L.) Maton, also known as the ‘Queen of Spices’ is a rhizomatous herbaceous monocot from the family Zingiberaceae. In the present study, using HiSeq™ 2000 RNA sequencing technology, transcriptome sequencing was performed for both control and disease stressed small cardamom leaf tissues. RNA-seq generated 46,931,637 (101 base) and 31,682,496 (101 base) raw reads and totally 9.93GB and 6.63GB of sequence data for cardamom control and stressed samples respectively. The raw data were submitted to NCBI SRA database of under the accession numbers SRX2512359 and SRX2512358 for the control and diseased samples respectively. The raw reads were quality filtered and assembled using TRINITY de novo assembler which created 1,11,495 (control) and 91,096 (diseased) contigs with N50 values 3013 (control) and 2729 (stressed). The data was further used to identify significantly differentially expressed unigenes between control and stressed samples. Assembled unigenes were further annotated and evaluated in silico to predict the function using publicly available databases and gene annotation tools. Keywords: Small cardamom, RNA sequencing, Transcriptome, Differential expression
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机译:小豆蔻(Elettaria Cardamomum(L.)Maton,也被称为“香料女王”是来自家庭Zingiberaceae的根茎草本单码。在本研究中,使用Hiseq™2000 RNA测序技术,对所述控制进行转录组测序和疾病强调了小豆蔻叶组织。RNA-SEQ分别产生的46,931,637(101个碱)和31,682,496(101个基础)原始读取和总共9.93GB和6.63GB的豆蔻控制和6.63GB的序列数据。原始数据已提交给NCBI SRX2512359和SRX2512358的SRA数据库分别用于控制和患病样本。原料读数是质量过滤并使用Trinity de Novo汇编程序组装,该组合物创建1,11,495(Control)和91,096(患病)Contig,其中N50值3013(对照)和2729(强调)。数据进一步用于鉴定控制和压力样品之间的显着差异表达的未成语。组装的unigenes在硅中进一步注释和评估,以预测使用公共数据库和基因注释工具的功能。关键词:小豆蔻,RNA测序,转录组,差异表达
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