Osteopontin () gene is a secreted phosphoprotein which appears to play a keyfunction in the conceptus implantation, placentation and maintenance ofpregnancy in pigs. The objectives of this study were to verify thenon-synonymous single nucleotide polymorphisms (SNPs) and their association with litter size traits incommercial Thai Large White pigs. A total of 320 Thai Large White sows weregenotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Three SNPs at c.425G> A,c.573T> C and c.881C> T revealed amino acid exchangerates of p.110Ala> Thr, p.159Val> Ala andp.262Pro> Ser, respectively, and were then segregated. Thesethree SNPs were significantly associated with total number born (TNB) andnumber born alive (NBA) traits. No polymorphisms of the two SNP markers(c.278A> G and c.452T> G) were observed in this study.Moreover, the SNPs at c.425G> A and c.573T> C werefound to be in strong linkage disequilibrium. The association of withlitter size emphasizes the importance of porcine as a candidate gene forreproductive traits in pig breeding.
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