The tissue attachment and wound healing in conjunctional transplantation was more rapid with autologous fibrin glue (AFG) than suture techniques. Both tissue attachment and wound healing process need interaction between fibronectin (FN) dan integrin α5 activating the intra cellular signal transduction pathway. The aim of this study was to evaluate the gene expression, i.e. FN and integrin in conjunctival transplantation, comparing between AFG and suturing techniques. Animal experimental study was done in New Zealand White rabbits, which divided into AFG and suturing technique at Laboratorium Sentral (Biologi Molekuler) FK Unpad Bandung during May–October 2008, each 8 rabbits, respectively. The tissue sample for reverse transcriptase polymerase chain reaction (RT-PCR) examination was taken from the tissue excision one day after conjunctival bulbi transplantation. Data analysis was tested using Mann Whitney for small sample. The FN gene expression power of messenger ribonucleic acid (mRNA) in the AFG technique was stronger than that in suturing technique (1.9 vs 1.0, p=0.014). There was no significant difference in integrin α5 gene expression of mRNA between AFG and suturing techniques (1.2 vs 1.0, p=0.235). In conclusions, FN gene expression in AFG technique is stronger than suturing technique. There is no difference in integrin α5 gene expression between two techniques, however there is a tendency of increased integrin α5 gene expression one day after surgery
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机译:组织附着和伤口愈合在结合移植中比缝合技术更快地与自体纤维蛋白胶(AFG)更快。组织附着和伤口愈合过程都需要纤连蛋白(FN)DAN整合蛋白α5之间的相互作用,激活细胞内胞内信号转导通路。该研究的目的是评估基因表达,即结膜移植中的整合蛋白,比较AFG和缝合技术。动物实验研究是在新西兰白兔进行的,分别在2008年5月10日5月10日,每8只兔子在实验室哨班(Biologhi Molekuler)FK Unpad Bandung的AFG和缝合技术。逆转录酶聚合酶链反应(RT-PCR)检查的组织样品从组织直接移植后一天取自组织切除。使用Mann Whitney进行数据分析,用于小样本。 AFG技术中信使核糖核酸(mRNA)的FN基因表达能力比缝合技术(1.9 Vs 1.0,P = 0.014)强。 EFG和缝合技术MRNA的整联α5基因表达没有显着差异(1.2 Vs 1.0,P = 0.235)。在结论中,AFG技术中的FN基因表达比缝合技术强。两种技术之间的整合蛋白α5基因表达没有差异,但是在手术后一天存在增加的整联蛋白α5基因表达趋势
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