Chemical composition and “ in vitro ” anti-inflammatory activity of Vitis vinifera L. (var. Sangiovese) tendrils extract

摘要

Grapevine (Vitis vinifera L.) is currently used for wine production, herbal medicine and foodudsupplements. In this study we investigated the phenolic composition of grape tendril extractud(TVV), because these compounds are probably responsible for the observed biological effectsudof TVV.We performed our investigation on cells implicated in the inflammatory process andudin vascular damage, such as monocyte (U937) and senescent endothelial (HUVEC) cells.Weudanalysed changes in microRNA (miRNA) expression during a pro-inflammatory conditionudin LPS (lipopolysaccharide) stimulated cells. MiR-126, defined as angiomiR for its ability inudthe maintenance of endothelial cell functions, and miR-146a, defined as inflamma-miR, forudits ability to down-modulate (NF-kB nuclear factor-kB) pro-inflammatory pathway, wereudanalysed along with their targets.TVV extract (40 microg/mL) was able to prevent the LPS stimulusudand may play an important role in the prevention of endothelial dysfunction when theudaccumulation of senescent endothelial cells occurs.